selective et b receptor antagonist  (Alomone Labs)

Journal: Molecular Pain

Article Title: Endothelin receptor type A is involved in the development of oxaliplatin-induced mechanical allodynia and cold allodynia acting through spinal and peripheral mechanisms in rats

doi: 10.1177/17448069211058004

Figure Lengend Snippet: Systemic administration of a dual ET A /ET B receptor antagonist prevented oxaliplatin-induced mechanical allodynia and cold allodynia: Mechanical allodynia (a, b) and cold allodynia (c) were examined using the von Frey test and the acetone test, respectively. Bosentan (50 mg/kg) was intraperitoneally administered for 8 consecutive days (A; from days −1 to 6) and for 2 consecutive days (b and c; days −1 and 0) before intraperitoneal oxaliplatin administration ( n = 6). (a) Mechanical allodynia was examined before each drug administration and 2 h, 8 h, 24 h, 4 days, 7 days, 11 days, and 14 days after oxaliplatin administration. (b) The dose–response relationship of bosentan was examined at day 1 after oxaliplatin administration. (c) Cold allodynia was performed before and 2 h, 8 h and 24 h after oxaliplatin administration. * p < 0.05 and ** p < 0.01, compared with the vehicle group using the Mann–Whitney U -test (a, c). ** p < 0.01, compared with the vehicle group using the Steel test (b). Pre: before administration of bosentan.

Article Snippet: Oxaliplatin (Yakult Corporation, Tokyo, Japan) was diluted in 5% glucose solution (1 mg/mL) and intraperitoneally administered at a dose of 5 mg/kg at day 0. , Bosentan, a dual ET A /ET B receptor antagonist (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), atrasentan, a selective ET A receptor antagonist (Sigma-Aldrich, St Louis, MO, USA) and BQ-788, a selective ET B receptor antagonist (Alomone Labs, Jerusalem, Israel) were dissolved in 60% dimethylsulfoxide and 40% propylene glycol.

Techniques: MANN-WHITNEY

Journal: Molecular Pain

Article Title: Endothelin receptor type A is involved in the development of oxaliplatin-induced mechanical allodynia and cold allodynia acting through spinal and peripheral mechanisms in rats

doi: 10.1177/17448069211058004

Figure Lengend Snippet: Systemic administration of an ET A receptor antagonist, but not an ET B receptor antagonist, prevented oxaliplatin-induced mechanical allodynia and cold allodynia: Mechanical allodynia (a, b, d) and cold allodynia (c, e) were examined using the von Frey test and the acetone test, respectively. An ET A receptor antagonist, atrasentan (a–c), or an ET B receptor antagonist, BQ-788 (d, e), was intraperitoneally administered for 2 consecutive days (days −1 and 0) before intraperitoneal oxaliplatin administration (5 mg/kg) on day 0 ( n = 5–8). (a, d) Mechanical allodynia was examined before each drug administration and 2 h, 8 h, 24 h, 4 days, 7 days, 11 days, 14 days, 21 days, and 28 days after oxaliplatin administration. (c, e) Cold allodynia was examined before atrasentan, BQ-788 and oxaliplatin administration and 2 h, 8 h, 24 h and 4 days after oxaliplatin administration. * p < 0.05 and ** p < 0.01, compared with the vehicle group using the Mann–Whitney U -test. (b) The dose–response relationship of atrasentan was examined at day 1 after oxaliplatin administration. * p < 0.05, compared with the vehicle group using the Steel test. Pre: before administration of atrasentan or BQ-788.

Article Snippet: Oxaliplatin (Yakult Corporation, Tokyo, Japan) was diluted in 5% glucose solution (1 mg/mL) and intraperitoneally administered at a dose of 5 mg/kg at day 0. , Bosentan, a dual ET A /ET B receptor antagonist (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), atrasentan, a selective ET A receptor antagonist (Sigma-Aldrich, St Louis, MO, USA) and BQ-788, a selective ET B receptor antagonist (Alomone Labs, Jerusalem, Israel) were dissolved in 60% dimethylsulfoxide and 40% propylene glycol.

Techniques: MANN-WHITNEY

Journal: Physiological Reports

Article Title: Endothelin inhibits renin release from juxtaglomerular cells via endothelin receptors A and B via a transient receptor potential canonical‐mediated pathway

doi: 10.14814/phy2.12240

Figure Lengend Snippet: RT‐PCR for ETA (top) and ETB (bottom) identifying bands at 260 and 231 bp, respectively. Column 1 is the “no‐template” negative control. Column 2 is total mRNA obtained from isolated juxtaglomerular (JG) cells. Column 3 is total mRNA obtained from the positive control of liver. Column 4 is the calibration scale (100 bp ladder).

Article Snippet: The cells were incubated for 1 h with either an ETA or an ETB antibody (Alomone, Jerusalem, Israel; Murphy et al. ) diluted 1:100 in 5% BSA.

Techniques: Reverse Transcription Polymerase Chain Reaction, Negative Control, Isolation, Positive Control

Journal: Physiological Reports

Article Title: Endothelin inhibits renin release from juxtaglomerular cells via endothelin receptors A and B via a transient receptor potential canonical‐mediated pathway

doi: 10.14814/phy2.12240

Figure Lengend Snippet: Immunofluorescence of a single juxtaglomerular (JG) cell using two antibodies; one specific for renin (in green) to confirm this is a JG cell, and another specific for the ETA (top) and ETB (bottom) receptors isoforms (in red). The third panel shows both renin and A1R in the same JG cell (merged image).

Article Snippet: The cells were incubated for 1 h with either an ETA or an ETB antibody (Alomone, Jerusalem, Israel; Murphy et al. ) diluted 1:100 in 5% BSA.

Techniques: Immunofluorescence

Journal: World Journal of Gastroenterology : WJG

Article Title: Gene expression profiling and endothelin in acute experimental pancreatitis

doi: 10.3748/wjg.v18.i32.4257

Figure Lengend Snippet: Mechanical and thermal hypersensitivity. A: Rats with pancreatitis demonstrate fewer withdrawal events in response to mechanical stimuli at time points after treatment with BQ123 [endothelin (ET)-A receptor antagonist, 300 μmol/L] and BQ788 (ET-B receptor antagonist, 300 μmol/L). The response to BQ788 persisted for a shortened amount of time compared to the longer lasting to BQ123. Data is presented as actual number of events (n = 5); B: Endothelin-A receptor antagonist (BQ123) normalized secondary thermal hypersensitivity that was shortened after induction of pancreatitis. The effect persisted longer for BQ123 compared to shorter response to BQ788 antagonist (n = 5). PBS: Phosphate buffered saline; DBTC: Dibutyltin dichloride.

Article Snippet: Polyclonal rabbit anti- ET-A and ET-B receptor (Alomone Labs, Jerusalem, Israel) were used for staining frozen sections of the DRG.

Techniques:

Journal: World Journal of Gastroenterology : WJG

Article Title: Gene expression profiling and endothelin in acute experimental pancreatitis

doi: 10.3748/wjg.v18.i32.4257

Figure Lengend Snippet: Pancreatic tissues from naïve and pancreatitic animals. A: Naïve endothelin (ET)-AR; B: Dibutyltin dichloride (DBTC) ET-A receptor; C: Naïve ET-B receptor; D: DBTC ET-B receptor. ET-A receptors are expressed in ducts (arrows) which appear more constricted in pancreatitis animals (B: DBTC ET-A receptor). ET-B receptors were expressed on the vasculature (4’,6-diamidino-2-phenylindole, nuclear blue counterstaining) 40× magnification (n = 5).

Article Snippet: Polyclonal rabbit anti- ET-A and ET-B receptor (Alomone Labs, Jerusalem, Israel) were used for staining frozen sections of the DRG.

Techniques:

Journal: World Journal of Gastroenterology : WJG

Article Title: Gene expression profiling and endothelin in acute experimental pancreatitis

doi: 10.3748/wjg.v18.i32.4257

Figure Lengend Snippet: Immunohistochemical analysis of the localization of endothelin-A and endothelin-B receptors in dorsal root ganglia from naïve or pancreatitic rats. A: Naïve endothelin (ET)-A; B: Dibutyltin dichloride (DBTC) ET-A; C: Naïve ET-B; D, E: DBTC ET-B (n = 5). ET-A receptor expression is shown in primary sensory neurons (A, B). In contrast, ET-B receptors (B-E) are primarily localized on the Schwann cells (myelin sheaths) surrounding the axons passing through the dorsal root ganglia from naïve or pancreatitic rats (4’,6-diamidino-2-phenylindole nuclear blue staining and Alexa Fluor 568, red).

Article Snippet: Polyclonal rabbit anti- ET-A and ET-B receptor (Alomone Labs, Jerusalem, Israel) were used for staining frozen sections of the DRG.

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: World Journal of Gastroenterology : WJG

Article Title: Gene expression profiling and endothelin in acute experimental pancreatitis

doi: 10.3748/wjg.v18.i32.4257

Figure Lengend Snippet: Endothelin receptor A and B in dorsal root ganglia (T10-12). A: Double staining of Endothelin receptor A with dual staining for neuronal (NeuN) and glial fibrillary acidic (GFAP) markers from naïve animal; B: Dual staining for Endothelin A and NeuN in a pancreatitic animal; C: NeuN in a pancreatitic animal; D: Endothelin receptor A in a pancreatitic animal. Significant increases in the endothelin (ET)-A are noted in dorsal root ganglia (DRG) neurons of all sizes on day 7 after induction of pancreatitis; E: Dual staining for Endothelin B and GFAP in a naïve animal; F: Dual staining for Endothelin B and GFAP in a pancreatitic animal; G: GFAP in a pancreatitic animal; H: Endothelin receptor B in a pancreatitic animal; Significant increases in the ET-B receptor are noted localized in satellite glia and Schwann cell myelin in thoracic DRG on day 7 after induction of pancreatitis. Alexa Fluor 568, red; Alexa Fluor 488, green (n = 5).

Article Snippet: Polyclonal rabbit anti- ET-A and ET-B receptor (Alomone Labs, Jerusalem, Israel) were used for staining frozen sections of the DRG.

Techniques: Double Staining, Staining

Journal: Pain

Article Title: Role of peripheral endothelin receptors in an animal model of complex regional pain syndrome type 1 (CRPS-I)

doi: 10.1016/j.pain.2010.07.003

Figure Lengend Snippet: Table 1

Article Snippet: Non-specific binding sites were blocked by incubation in 5% skim milk for 30 min. Membranes were incubated overnight at 4°C in rabbit-derived anti-ETA-R or ETB-R (1:200, Alomone, Jerusalem, Israel).

Techniques: